Genetics Research has developed two forms of enrichment by Negative Selection. These two approaches can be combined with each other and other existing techniques for a myriad of sample preparation applications.
“Resistance” integrates chemical modifications into the termini of selected DNA loci; these modifications resist exonuclease degradation while the unwanted balance of the DNA sample is eliminated.
“Protection” uses CRISPR/Cas 9 complexes to identify and bind to the termini of selected DNA loci. The CRISPR/Cas 9 complexes serve as steric blocks to exonuclease proteins, protecting the loci from exonuclease action while the unwanted remainder of the sample is destroyed.